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. 2003 Apr;77(7):4070–4080. doi: 10.1128/JVI.77.7.4070-4080.2003

FIG. 5.

FIG. 5.

Effect of cell surface DC-SIGN and DC-SIGNR expression levels on HIV-HCV pseudotype binding. DC-SIGN, DC-SIGNR, and control T-REx cells were induced overnight with the indicated concentrations of doxycycline, and receptor expression and binding of HC-J4 E2/SIN viruses were assessed in parallel. (A) Surface expression of DC-SIGN and DC-SIGNR. DC-SIGN- and DC-SIGNR-positive cells were incubated in medium containing the indicated concentrations of doxycycline, stained with anti-AU-1 antibody, and analyzed by FACS. (B) HC-J4 E2/SIN pseudotype binding to DC-SIGN, DC-SIGNR, and control cells. The indicated cell lines were incubated with equal volumes of medium containing 1.5 μg of p24 antigen-normalized virus, and binding was quantified as described in Materials and Methods. The percentage of bound p24 antigen is indicated. A representative experiment carried out in triplicate is shown, and the standard deviation is indicated. Similar results were obtained in an independent experiment.