FIG. 6.

Binding of HIV-HCV pseudotype particles to HUVECs transduced to express DC-SIGN, DC-SIGNR, and CCR-5. HUVECs were infected overnight with lentiviruses encoding DC-SIGN, DC-SIGN and DC-SIGNR, and CCR-5 and maintained for 3 days as previously described (59a). The level of DC-SIGN and DC-SIGNR expression was similar to that obtained with T-REx cells (data not shown). Thereafter, binding of 2 ng of p24 antigen-normalized HC-J4 E1/SIN and E2/SIN pseudotype viruses was assessed as described in Materials and Methods. The percentage of recovered viral p24 antigen is indicated. A representative experiment carried out in quadruplicate is shown, and the standard deviation is indicated. Similar results were obtained in an independent experiment.