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. 2001 Sep;3(5):411–419. doi: 10.1038/sj.neo.7900175

Figure 3.

Figure 3

Blocking Chk1 expression induces cell death. H1299 cells were transfected with different plasmids as indicated using LipofectAMINE 2000. (A) Chk1 protein levels in the transfected cells. Lane 1: vector control; lane 2: full-length Chk1 sense cDNA; lane 3: full-length Chk1 antisense cDNA; lane 4: ribozyme 910. (B) FACS analysis of the transfected cells. H1299 cells were transfected with different plasmids as indicated. Twenty-four hours after transfection, both the floating and the attached cells were harvested and subjected to FACS analysis. (C) The quantification of the FACS analysis. (D) Caspase assay. H1299 cells were transfected with different plasmids as indicated. Fourty-eight hours after transfection, caspase activity was measure as described in the Materials and Methods section.