TABLE 3.

Oligonucleotide binding by HCV helicase^a

Helicase	dCT9		dGA9
	KD (nM)	ΔFMAX	KD (nM)	ΔFMAX
Hel-1a	16 ± 3	0.41	71 ± 25	0.30
Hel-1b	5 ± 1.5	0.39	36 ± 6	0.23
Hel-2a	1.6 ± 0.3	0.32	2.8 ± 0.5	0.32
T450I	13 ± 4	0.34	9.1 ± 4	0.25

^aThe maximum change in intrinsic protein fluorescence (ΔF_MAX) and apparent dissociation constant (K_D) were determined by fitting the data shown in Fig. 6 to equation 5.