FIG. 2.

Transient complementation of the UL52 null virus hr114. Complementation abilities of zinc finger mutants were determined as described in Materials and Methods. The complementation index of wild-type UL52 was set at 100%, which represents an approximate 100-fold increase in viral yield with the wild type over that with noncomplementing mutants. The complementation index was obtained by dividing the titer of virus produced in cells transfected with wild-type or mutant versions of UL52 plasmids and superinfected with hr114 by the titer in supernatants from mock-transfected cells. Data presented are averages from three or more assays performed with each mutant. The mean percent complementation index is shown. Error bars reflect the standard deviations calculated from the independent complementation assays performed.