FIG. 6.

PML recruitment to stage III foci. HEp2 cells were transfected with plasmids bearing wild-type or mutant versions of UL52 and then superinfected with hr114 for 5.5 h p.i. Cells were stained with the polyclonal αICP8 antibody and the monoclonal anti-PML antibody PG-M3. The secondary antibodies were goat anti-rabbit immunoglobulin conjugated to FITC for ICP8 and goat anti-mouse immunoglobulin conjugated to Texas red for PML. Staining was visualized by fluorescence microscopy. (A through C) Cells were transfected with the CC3,4AA mutant and stained with either ICP8 (A) or PML (B); the images were merged (C). (D through F) Cells were transfected with the R1002A mutant and stained with either ICP8 (D) or PML; the images were merged (F).