Table 1.

Subcellular fractionation of kinesin-related proteins in undifferentiated myoblasts and differentiated myotubes

	KIF3 Family						Other KRPs				Control
	3A PM	3A DM	3B PM	3B DM	3C PM	3C DM	K PM	K DM	1C PM	1C DM	NK PM	NK DM
P1 (3,000-g pellet)	14	14	12	13	16	25	24	14	21	24	41	45
P2 (9,000-g pellet)	12	12	12	11	15	18	17	19	23	12	40	40
P3 (100,000-g pellet)	32	29	35	25	29	27	20	28	43	24	14	10
S3 (100,000-g sup.)	42	45	41	51	40	30	39	39	13	40	5	5
P3 buffer wash	35	48a	43	50a	43	57a	40	42	76	80	72	72
S3 buffer wash	65	52	57	50	57	43	60	58	24	20	28	28
P3 Tx100 wash	30	51a	35	54a	41	53a	48	34	66	82	19	19
S3 Tx100 wash	70	49	65	46	59	47	52	66	34	18	81	81

PM, proliferating myoblasts; DM, differentiating myotubes; 3A, 3B, 3C = KIF3A-C respectively; K, kinesin heavy chain; 1C, KIF1C; NK, Na⁺K⁺-ATPase. 

Numbers are percentages of total Western band intensity within a particular fractionation. 

^a Note shift of Kif3 family proteins from S3 to the nondetergent-extractable P3 pellet upon differentiation.