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Specificity ELISA. Comparison of 2F5IgA, 2F5IgM, and 2G12IgM CHO supernatants with 2F5IgG and 2G12IgG. Antibody concentrations (in nanograms/milliliter) are plotted against optical density (OD) values. For the specificity ELISA, we used the HIV-1 envelope antigens ELDKWA (amino acids 662 to 667) (A), gp160 (B), and gp120 (C) and a mouse anti-2G12 idiotype antibody (D). The samples were detected by anti-human κ light chain AP conjugate.
