TABLE 2.
Neutralization values for antibodies 2F5 and 2G12 against primary HIV-1 isolatesa
| Isolate | % EC | Result (μg/ml) for antibody:
|
||||
|---|---|---|---|---|---|---|
| 2G12IgG | 2G12IgM | 2F5IgG | 2F5IgM | 2F5IgA | ||
| P7/366 dual tropic | 50 | >50 | 1.1 | >50 | 2.3 | 36.5 |
| 90 | >50 | >25 | >50 | >40 | >40 | |
| 99 | >50 | >25 | >50 | >40 | >40 | |
| S2/04 M-tropic | 50 | 22.8 | 3.6 | 1.7 | 3.2 | >40 |
| 90 | >50 | >25 | 18.6 | 33.7 | >40 | |
| 99 | >50 | >25 | >50 | >40 | >40 | |
| P3/366 M-tropic | 50 | >50 | 0.7 | 35.2 | 8.3 | >40 |
| 90 | >50 | 16.9 | >50 | >40 | >40 | |
| 99 | >50 | >25 | >50 | >40 | >40 | |
| P2/71 M-tropic | 50 | >50 | 2.9 | 5.6 | 0.1 | 4.0 |
| 90 | >50 | 7.1 | 15.4 | 10.6 | 10.0 | |
| 99 | >50 | 23.3 | 25.2 | 19.2 | 19.1 | |
| S2/03 | 50 | NT | NT | 2.9 | >50 | 22.7 |
| 90 | NT | NT | 9.5 | >50 | >40 | |
| 99 | NT | NT | 21.2 | >50 | >40 | |
| 92/BR/30 M-tropic B clade | 50 | 5.0 | 0.5 | 15.1 | >50 | NT |
| 90 | >50 | 1.5 | 41.6 | >50 | NT | |
| 99 | >50 | 5.9 | >50 | >50 | NT | |
| 92/UG/29 T-tropic A clade | 50 | 46.7 | 1.62 | 16.1 | >50 | NT |
| 90 | >50 | 7.86 | 24.6 | >50 | NT | |
| 99 | >50 | >20 | >50 | >50 | NT | |
| 92/UG/37 M-tropic A-clade | 50 | 0.2 | 0.7 | 3.9 | >50 | >40 |
| 90 | 49.7 | 4.7 | 9.6 | >50 | >40 | |
| 99 | >50 | >20 | 22.9 | >50 | >40 | |
a
The inhibition of virus production was assessed by measuring p24 concentrations in HIV-infected PBMC cultures. The neutralization titers of the antibodies were calculated. The EC50s, EC90s, and EC99s represent the concentrations of antibody (micrograms/milliliter) causing a 50, 90, and 99% reduction of HIV-infected PBMC. No neutralization is indicated by an EC of >50, >40, or >25 μg/ml, each representing the maximal antibody concentration used in the assay. NT, not tested.