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. 2000 Dec;11(12):4159–4171. doi: 10.1091/mbc.11.12.4159

Figure 3.

Figure 3

Two coilin truncation mutants localize to the nucleolus and CBs, but do not mis-localize endogenous coilin, snRNPs, or SMN to the nucleolus. (A) GFP-coilin(1–248) and GFP-coilin(1–315) mutants, upon transfection into HeLa, were found to have identical nucleolar and CB expression profiles. Antibodies to fibrillarin and Nopp140, which normally localize in nucleoli and CBs, reveal complete colocalization with these proteins. Endogenous coilin localization was determined in GFP-coilin(1–248) transfected cells using anticoilin antibodies that recognize epitopes downstream of the truncation. Note that endogenous coilin is not mislocalized to the nucleolus in GFP-coilin(1–248)transfected cells. (B and C) GFP-coilin(1–248) transfected cells were assessed for the localization of Sm proteins (B) and SMN (C). No nucleolar accumulations of snRNPs or SMN were observed. As expected, accumulations of snRNPs could be detected in the CBs (arrows). Likewise, SMN is present in the CB foci of GFP-coilin(1–248) transfected cells (our unpublished results). However, a small fraction of GFP-coilin(1–248) cells did not display CBs, yet SMN foci, or gems, are present in these cells (C).