Figure 7.

Coilin mutational summary. The localization of full-length coilin (1–576) and coilin mutants generated in this study are described. The putative coilin nucleolar localization signal (NoLS), nuclear localization signals (NLS, dark boxes), self-interaction, CB localization, and nucleic acid binding domains are indicated. The NLS in the coilin(1–92) construct was added exogneously, along with GFP- and myc-tags. Positive signs (+) denote basic regions of coilin, whereas negative signs (−) indicate potentially-acidic serine patches from amino acids 242–259 and 312–325 (stippled boxes). Two additional coilin mutants, myc-coilin(1–234) and myc-coilin(1–291) were generated by Bohmann et al. (1995).