FIG. 2.

(A) In vitro processing of wild-type or mutated polyprotein pp62 forms by the ASFV protease. As a control, the translated products were incubated in the absence of protease (C−) or in the presence of the catalytically inactive protease (pC232S) for 1 h. In incubations for up to 24 h with the mutant protease, no processing of the polyprotein was observed (data not shown). The reactions were carried out for the indicated times at 30°C. The intermediate and final processing products are indicated. (B) Representation of the percentage of unprocessed precursor at the time points indicated. The pp62- and pp220-derived fragments containing only one cleavage site were compared to the full-length polyprotein pp62.