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Optimization of a HeLa cell in vitro translation system. Effect of potassium acetate (KOAc, A) on translation of Renilla (rLuc) and firefly luciferase (fLuc) ORFs (first and second cistrons, respectively). RLU, relative light units. (B) RNA dose-response. The RNA contains the full-length HIV-1 5′ leader (nucleotides 1 to 336).
