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Demarcation of the HIV-1 IRES. Renilla luciferase (rLuc) and firefly luciferase (fLuc) activity (left and right panels, respectively) in HeLa cells 28 h after transient cotransfection with the LacZ reporter and HIV-1 constructs. The results are from a representative experiment performed in triplicate and are expressed as stimulation of translation relative to the control vector. The results did not vary by more than 10%.
