FIG. 5.

HIV-1 IRES-driven translation functions with proteolytically cleaved eIF4G. Monocistronic capped RNAs from HIV-1, encephalomyocarditis virus (EMCV), and cyclin B2 (A) were in vitro translated in a rabbit reticulocyte lysate treated with the foot-and-mouth disease virus L protease as previously described (52, 53, 62, 64). [³⁵S]methionine-labeled proteins were subjected to SDS-PAGE and quantified (B) as described in Materials and Methods.