FIG. 6.

Increased secondary structure in the primer-binding site region does not inhibit translation. C33A cells were transfected with the wild-type or mutant proviral constructs shown in panel A. Total cellular extracts were prepared 3 days posttransfection, and viral proteins were detected by Western blotting with serum from an HIV-1-infected individual (B). The positions of the HIV-1 gag-p55 precursor protein and the mature CA-p24 protein are indicated on the right. Positions of molecular size markers are indicated on the left (in kilodaltons).