FIG. 7.

HIV-1 IRES is active in translation extracts prepared from mitotic cells. FACS analysis of untreated (A) and nocodazole-treated (B) HeLa cells from which in vitro translation extracts were prepared.In vitro translation of bicistronic capped mRNA in untreated (□) and nocodazole-treated (▪) HeLa cell extracts (C and D). The values are averages from two independent experiments. The difference in the translation efficiencies of the extracts was normalized by setting the firefly luciferase activity of the negative control vector plasmid to an equal value. See the legend to Fig. 2 for abbreviations.