FIG. 8.

HIV-1 IRES functions ex vivo in G₂/M-arrested HeLa cells. FACS analysis of untreated (A) and nocodazole-treated (B) HeLa cells which express Plap/neo as described in Materials and Methods. (C) [³⁵S]methionine-labeled proteins were resolved by SDS-PAGE and visualized by autoradiography. (D) Western blotting for neomycin and actin proteins was performed as described in Materials and Methods. Experiments were repeated three times, and the results of a representative experiment are shown. The results did not differ by more than 20%.