FIG. 5.

Cytoplasmic accumulation of ICP27 is inhibited by DRB. (A) BHK cells were mock infected or infected with wt HSV-1 and were fixed 7 h after infection. Cells remained untreated, or DRB (5 μM) was added with virus. Immunofluorescence staining was performed with the 1113 monoclonal antibody for ICP27, with a monoclonal antibody for the cellular protein hnRNPA1, and with the polyclonal β-c antibody for CK2. (B) HSV-induced cytoplasmic redistribution of CK2 is inhibited by DRB. BHK cells were infected with wt HSV-1 or with different ICP27 viral mutants. The example shown is that of the d2-3 virus, which redistributes CK2 to the cytoplasm in the absence of DRB.