FIG. 2.

CpG methylation pattern of the LTR region in a Jurkat clonal cell line latently infected with an LTR-Tat-IRES-GFP HIV-1-derived vector before and after reactivation of HIV-1 with 10 nM PMA. (A) Positions of CpG in the HIV-1 LTR. The arrow at the U3-R junction denotes the start site of transcription (nucleotide +1). (B) Bisulfite sequencing of HIV-1 promoters from top to bottom. (a) Nonstimulated, latently infected cells; (b) 10 nM PMA-stimulated cells, GFP-negative cell sorting; (c) 10 nM PMA-stimulated cells, GFP-positive cell sorting. Open circles, nonmethylated CpG residues; closed circles, methylated CpG residues. The total percentages of methylated CpG (mCpG) for nonstimulated and PMA-stimulated cells (GFP negative and GFP positive) are indicated in panel B.