Figure 3.
Bcl-2 associates with Cdc2 in cells arrested with antimicrotubule drugs. Detergent-solubilized lysates (2x106 cells/sample) were prepared from 293-Bcl-2 cells cultured without treatment (lanes 1 and 4), ∼2 µM nocodazole (lanes 2 and 5) or nocodazole and 1 µM flavopiridol (lanes 3 and 6). Samples were either analyzed directly by immunoblotting with anti-Cdc2 antibody (lower panel) or subjected to immunoprecipitation with anti-mIgG (lanes 1–3) or with anti-Bcl-2 antibodies (lanes 4–6) followed by SDS-PAGE/immunoblot analysis with anti-Cdc2 antibody (upper panel).