Figure 5.

Subcellular localization of Bcl-2 in control and M-phase-arrested cells. 293-Bcl-2 cells were either cultured without treatment (lanes 1–4) or arrested by treatment with ∼2 µM nocodazole (lanes 5–8) for ∼16 hours. Subcellular fractions were prepared, including HM (heavy membrane), LM (light membrane), N (nuclear), and C (cytosolic) fractions. Fractions were normalized for cell equivalents (10⁷ cells each) and subjected to SDS-PAGE/immunoblot analysis using anti-Bcl-2 antibody with ECL-based detection. Reprobing the same blot with an antibody specific for mitochondrial F₁β-ATPase confirmed successful fractionation (not shown).