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. 2001 Nov;3(6):521–526. doi: 10.1038/sj.neo.7900187

Figure 1.

Figure 1

Semiquantitative duplex PCR optimization of SGC31117 and GAPDH primers. (A) Exponential range of amplification was detected by analyzing PCR products for duplexed primer sets after completion of cycles 22, 24, 26, 28, 30, 32, and 35. The products were resolved on an ethidium bromide-stained 3% agarose gel. Band intensities were quantified by densitometry. (B) Log (peak area) versus cycle number graph reveals the linear range of amplification. For these primers, 24 cycles were chosen for semiquantitative duplex PCR screening of breast cancer cell lines for amplification.