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. 2001 Nov;3(6):521–526. doi: 10.1038/sj.neo.7900187

Figure 3.

Figure 3

Amplification and overexpression of a gene (FLJ20128) that encodes a protein of unknown function distal to TBX2. (A) Semiquantitative duplex PCR for FLJ20128. Amplified samples are shown with asterisks. The ratios of amplification compared to the normal human control (C) is MCF7: 3.4, MDA-MB361: 2.5, SUM-52: 4, and T-200: more than 20-folds of amplification (exact ratio was not measured due to high amplification and saturation). (B) Semiquantitative duplex RT-PCR for FLJ20128. The ratios of overexpression compared to HPV11-21 is MCF7: 10, MDA-MB361: 4.5, SUM-52: 1.6, and T-200: 4.5-folds. HPV11-21 is a human papilloma virus immortalized nontumorigenic mammary cell line and is used as a control.