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. 2001 Nov;3(6):480–488. doi: 10.1038/sj.neo.7900204

Figure 3.

Figure 3

The dynamics of the dcmNFATtgn reporter system response. The FACS profiles of TKGFP reporter gene and CD69 expression in Jurkat/dcmNFATtgn cells clone 4 under nonstimulated basal conditions and after continuous stimulation with anti-CD3 alone or in combination with anti-CD28 antibodies. The percentage of gated cells in each quadrant is indicated (A). IL-2 production under nonstimulated basal conditions, and after 24-hour stimulation with anti-CD3 alone or in combination with anti-CD28 (B). The radiotracer FIAU/TdR accumulation ratio before and after stimulation with anti-CD3/CD28 antibodies in Jurkat/wild type (blank), Jurkat/dcmNFATtgn clone 3 (shaded), clone 4 (solid) (C).