Figure 2.

Bromelain inhibits glioma cell migration. (A) Cell migration (48 hours) from multicellular spheroids from different human glioma cell lines in 96-wells with and without bromelain (50 µg/ml). Bar, 100 µm. (B) Time lapse study of cells from an AN1/lacZ spheroid, which had been allowed 48 hours of migration in DMEM supplemented with 10% serum, before the medium was changed to a medium containing 50 µg/ml bromelain. The time points refer to the time after bromelain was added to the medium. *This picture was taken 96 hours after the bromelain medium was replaced with ordinary serum supplemented medium. Bar, 100 µm.