Figure 1.

NFκB mediates promatrilysin expression in LNCaP cells. LNCaP cells were transiently transfected with a plasmid construct encoding a minimal HIV promoter containing 2X NFκB binding elements driving a luciferase reporter gene. Transfected cells were treated with either PDTC (125 or 250 nM), sulfasalazine (0.5 or 1.0 mM), SN50 NFκB inhibitory peptide, or SN50B inactive control peptide (18 µM) 1 hour before stimulation with IL-1β. Twenty-four hours following IL-1β stimulation, (A) whole cell lysates were collected and analyzed for luciferase expression and (B) promatrilysin expression in conditioned media was quantified using ELISA analyses. The results shown represent the means and standard deviations of three experiments each performed in triplicate.