Figure 2.

Cyclohexamide blocks IL-1β-induced matrilysin mRNA. LNCaP cells were serum starved for 16 hours then were treated with either 200 pg/ml IL-1β (lane 1), serum-free DMEM (lane 2), or IL-1β (200 pg/ml) and cyclohexamide (10 µg/ml) simultaneously (lane 3). Total RNA was collected 8 hours following IL-1β stimulation. RNA samples were subjected to 1% agarose gel electrophoresis and transferred and crosslinked to a nylon membrane. The membrane was probed for matrilysin mRNA. Blots were stripped and reprobed for GAPDH (lower panel). The blot shown is representative of five repeats of this experiment.