Figure 4.

IL-6 induces promatrilysin expression in LNCaP cells. LNCaP cells were transiently transfected with a plasmid construct encoding a minimal HIV promoter containing 2X NFκB binding elements driving a luciferase reporter gene. Transfected cells were treated with escalating doses of recombinant IL-6. Twenty-four hours following cytokine stimulation, (A) conditioned media were analyzed for matrilysin expression using ELISA analyses and (B) luciferase expression in whole cell lysates was quantified. The results shown represent the means and standard deviations of at least three experiments each performed in triplicate.