Figure 7.

STAT3 mediates IL-6-induced promoter activity. LNCaP cells were transiently co-transfected with a plasmid construct encoding 1.1 kb of the human matrilysin promoter driving a luciferase reporter gene and, either a plasmid construct encoding a dominant negative STAT3 containing point mutations at critical phosphorylation sites, or pCMV-1, the parent vector into which the STAT3 double mutant was cloned. Transfected cells were treated with either IL-1β or IL-6, then luciferase activity in whole cell lysates was quantified 24 hours following cytokine stimulation. The results shown represent the means and standard deviations of at least three experiments.