FIG. 4.

Growth of recombinant viruses rTGEV-Δ3 and rTGEV-Δ3-TRS3a-GFP in swine. Groups of 14 2- to 4-day-old piglets were oronasally and intragastrically inoculated with 2 × 10⁸ PFU/animal of rPUR-MAD-SC11, rTGEV-Δ3, or rTGEV-Δ3-TRS3a-GFP. (A) Number of surviving piglets at different times postinoculation. Results of a representative experiment of two that gave similar results are shown. The significance of the results was analyzed by the Student t test (see Results section). (B) Growth of rPUR-MAD-SC11, rTGEV-Δ3, and rTGEV-Δ3-TRS3a-GFP viruses in the indicated tissues. Values are the means for representative tissue samples from three animals sacrificed 2 days after inoculation. Error bars represent the standard deviation.