FIG. 5.
Lactogenic immune response induced in swine by recombinant rTGEV-Δ3-TRS3a-GFP virus. Two pregnant sows seronegative for TGEV were immunized with the recombinant virus rTGEV-Δ3-TRS3a-GFP. The virus (2 × 108 PFU per animal) was administered by the nasal and intramuscular routes at days 35 and 75 of gestation. Serum from the sows and their progeny was collected at days 2, 7, 19, and 27 after delivery. Colostrum was collected from sows on the day of delivery. The antibody response against TGEV (A1, B1, and C1) and GFP (A2, B2, and C2) in the serum of the sows (A) and the progeny (B) and in the colostrum (C) was evaluated by ELISA. Values represented are the maximum level detected in the serum of sows (days 19 and 27 after delivery) and in the serum of the progeny (day 2 after delivery).