FIG. 1.
Inhibition of HIV-1 replication in hIgG-activated MDM. Open symbols, MDM (105 cells/well) were seeded in microtiter plates coated with 100 μl of a 1-mg/ml hIgG solution, resulting in ≈2 μg of bound hIgG/well. Closed symbols, uncoated control plates. For infection experiments, stimulated or control MDM were inoculated with R5 isolate HIV-1BaL (50 TCID50/106 cells) (A) or HIV-1BX08 (40 TCID50/106 cells) (B) or with X4 isolate HIV-1NL4-3 (2.103 TCID50/106 cells) (C) or HIV-1J34 (50 TCID50/106 cells) (D). HIV-1 replication was monitored by measuring p24 antigen in culture supernatants at days 4, 7, 10, and 14 or until day 28 for HIV-1J34. Results are presented as the means of results from four independent replicates ± standard deviations.