FIG. 8.
Analysis of transcripts and nuclear HIV-1 DNA in FcγR-stimulated or control MDM. MDM (106 cells/well of a 12-well plate) were activated with hIgG and immediately infected with HIV-1BaL (5 × 102 TCID50/106 cells) for 1 h at 37°C. Cells were lysed at the indicated time points after infection, and 5 μl of cell lysate (corresponding to approximately 20,000 cells) was analyzed by PCR. Primers specific for intermediate (pol) and late (U3/gag) reverse transcripts, nuclear 2LTR circles, or integrated provirus (Alu-LTR) were used. PCR products were analyzed by gel electrophoresis. Four independent experiments performed with MDM from different donors showed similar PCR amplification profiles despite signal intensity variations. NI, noninfected; −, absence of hIgG; +, presence of hIgG.