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. 2003 Apr;77(7):4205–4220. doi: 10.1128/JVI.77.7.4205-4220.2003

FIG. 5.

FIG. 5.

Hierarchical clustering of KSHV gene expression induced by Rta. (A) Color-coded expression scale. Expression of KSHV genes was strongly induced by Rta up to 48-fold by 24 h. (B) Color scale representing the degree of expression based on a calibrated ratio of Dox-stimulated Cy3-dUTP-labeled viral cDNA signal intensity over empty vector control Cy5-dUTP-labeled cDNA signal intensity. A calibrated ratio of 1, represented by black, indicates no change in expression. A calibrated ratio much greater than 1, represented by red, indicates a very strong increase in expression. Note the early increase in expression after 2 h of Rta induction (ORF 50), indicated by the arrowhead. While all genes were strongly overexpressed following Rta induction, it is apparent that certain genes show increases in expression earlier than others. The top scale is log 2 (CalRatio).