Figure 5.

Effect of mβCD and mβCD/chol on the Rab9-dependent pathway to the Golgi apparatus. (A) Representative example of the resulting bands seen after fluorography in a given experiment. The HeLa-TetOn/Rab9/M6PR46HMY cell line was grown for 3 d in sulfate-free medium. The cells were then treated with 5 mM mβCD or mβCD/chol before labeling of the modified M6PR with Na₂³⁵SO₄ for 2 h. The sulfated receptor was harvested and analyzed as described in Materials and Methods. The intensities of the resulting bands were determined by densiometric quantitation using ImageQuant 5.0. (B) Summary from two independent experiments.