Figure 6.

Effect of mβCD and mβCD/chol on the recycling (A) and degradation (B) of ricin in the HeLa-TetOn/Rab9S21N cell line. The cells were washed twice with HEPES medium, and incubated with or without 5 mM mβCD and mβCD/chol for 30 min at 37°C. ¹²⁵I-labeled ricin was then added, and the cells incubated further for 20 min before the amount of recycled and degraded ricin was measured as described in Material and Methods. The error bars show deviations between duplicates of a typical experiment.