FIG. 2.
Cytotoxicity on transgenic A2Kb mice immunized with 100 μg of pcDNA-IE1 and 100 μg of pcDNAGM-CSF (M1 to M4); 100 μg of pcDNA-IE1 and 100 μg of pcDNApp65mII (M5 to M8); and 50 μg of pcDNA-IE1, 50 μg of pcDNApp65mII, and 50 μg of pcDNAGM-CSF (M9 to M12). (A and B) Responses in spleen cells collected at day 10 after the fourth immunization. (C and D) Long-term memory responses in spleen cells collected at day 60 after the third immunization. The percent chromium release is reported at an effector/target ratio of 10:1, 30:1, or 100:1. Blasts and target cells were incubated with a mixture of five nonapeptides (25 μM each) derived from the IE1 protein for all panels except panel B, in which pp65p495 was used.