FIG. 3.

Immunodominant IE1 epitopes in A2Kb mice. Splenocytes from mice immunized with IE1 and GM-CSF DNA were stimulated in vitro with a peptide mixture and used as effector cells. (A) For preferential recognition of peptide in individual mice (M1 to M7), the specificity of the stimulated population was determined by Cr release with T2 cells sensitized with each individual peptide (100 μM) as targets (effector/target ratio of 100). (B) For preferential evaluation of each peptide, the IE1-297 peptide is shown as the most frequently recognized epitope in the mixture.