TABLE 1.

Cytokine flow cytometry with fresh blood from CMV-seropositive stem cell transplantation subjects stimulated with either the pp65 epitope or the IE1-297 epitope

UPNa	Day posttransplant	pp65p495		IE1-297		PHA,d % CD8+/IFN-γ
		% CD8+/IFN-γ	Total no. of cells/literb	% CD8+/IFN-γ	Total no. of cells/liter
100	40	0.11	4.08 × 105	0	0	2.09
93	120	3.62	4.77 × 107	0.16	2.11 × 106	37.6
70	150	0.47	NAc	0.20	NA	33.15
70	180	1.26	6.97 × 106	0.01	5.53 × 104	19.2

^aUnique patient number.

^bTotal number of IFN-γ-positive cells was calculated taking into account the percentage of total lymphocytes in the blood and the percent CD8⁺ cells from that fraction and expressed as number of cells per liter of blood. A total of 50,000 events were counted for each sample. The human immunodeficiency virus-negative controls (range, 0.03 to 0.31%) were subtracted from the reported numbers.

^cNA, total leukocyte count and percent lymphocyte count not available.

^dPHA, phytohemagglutinin.