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Analysis of the interaction of SITAC with the C-terminus of different transmembrane proteins. (A) C-terminal amino acid sequence of proteins containing the sequence Y-X-V/C-COOH. (B) Yeasts expressing the indicated C-termini fused to the GAL4 DBD were transformed with SITAC fused to the GAL4 AD and assayed for growth in -His/-Ade plates or for β-galactosidase activity. The histograms are the averages of triplicate determinations.
