Figure 4.

Effect of CA-rop2 and DN-rop2 Expression on the Development of Different Stages of Leaf Epidermal Pavement Cells.

Expanding leaves (∼0.5 cm in length) from wild-type plants and transgenic plants expressing CA-rop2 or DN-rop2 were used for this analysis. Cell shapes were imaged by confocal microscopy using plants expressing the GFP-tubulin fusion gene as described in the text.

(A) to (C) Wild-type epidermal cells expressing GFP-tubulin.

(D) to (F) CA-rop2 epidermal cells expressing GFP-tubulin.

(G) to (I) DN-rop2 epidermal cells expressing GFP-tubulin.

(A), (D), and (G) show pavement cells in stage I; (B), (E), and (H) show pavement cells in stage II; and (C), (F), and (I) show pavement cells in stage III. WT, wild type. Bar in (A) = 20 μm for (A) to (I).