Expression of OsTBP2 and OsTFIIB in E. coli.
(A) OsTBP2.
(B) OsTFIIB.
Lane 1, Ni affinity-purified fraction; lanes 2 to 4, sequential washes from the S-protein affinity matrix; lane 5, recombinant protein after excision of the S tag. Lane M, Molecular mass markers.