Figure 7.

Physical and Functional Interactions between OsTBP2 and the RF2a bZIP Transcription Factor.

(A) Functional interactions between RF2a and OsTBP2 in the transcription of the RTBV::uidA template by rice whole-cell extracts. Recombinant proteins (100 ng) were added as indicated 10 min before the initiation of the transcription reaction by addition of the whole-cell extract.

(B) Effect of OsTBP2 on binding complexes formed between RF2a and box II of the RTBV promoter.

(C) Protein gel blot analysis. Recombinant OsTBP2 (lane 1), coat protein of Tobacco mosaic virus (lane 2), and BSA (lane 3) were subjected to SDS–12% PAGE, blotted onto a nitrocellulose membrane, and incubated with recombinant RF2a. RF2a binding was visualized by alkaline phosphatase activity after sequential incubation of the membrane with rabbit anti-RF2a antibody and alkaline phosphatase–conjugated goat anti-rabbit IgG.