Figure 2.

Functional Complementation of a Yeast Mutant Deficient in Allantoin Uptake.

The dal4 dal5 mutant was transformed with the empty vector pFL61 (A) or with pFL61 carrying AtUPS1 (B). Three cell dilutions (1:1, 1:10, and 1:100) were plated on minimal medium containing 0.1% allantoin as sole nitrogen source and incubated for 5 days at 28°C.