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Expression of AtUPS1 mRNA in Different Plant Organs (A) and in Whole Plants Supplied with Different Nitrogen Sources (B).
RNAs were extracted and converted to cDNA by reverse transcriptase, and a 288-bp AtUPS1 fragment was amplified by 25 PCR cycles. To ensure that equal amounts of cDNA were added to each PCR reaction, an Actin2 fragment was amplified simultaneously by 20 PCR cycles (Act2).
