Figure 3.

Immunogold double labeling of endomannosidase and p58 in ultrathin frozen sections of clone 9 cells. Immunolabeling for p58 (large gold particles marked by arrows) is present in the intermediate compartment and one or two cis-cisternae, whereas endomannosidase (small gold particles) is additionally observed in medial cisternae (A and B). In the peripheral cytoplasm shown in C and D, vesiculotubulo clusters exhibit labeling for both p58 (large gold particles marked by arrows) and endomannosidase (small gold particles). Bars, 120 nm (A), 130 nm (B), 150 nm (C), and 100 nm (D).