Table 2.
PLA2 Activity Measured in Intact Cells by Fluorescence Microscopy (see Figure 10)
| Experiment | Increase of Fluorescence (Intensity Units ± sd, n) |
|---|---|
| Control | 18.1 ± 3.5 (n = 7) |
| Elicitor | 29.4 ± 5.0 (n = 7) |
| Control + ETYA | 3.8 ± 0.2 (n = 6) |
| Elicitor + ETYA | 4.0 ± 0.1 (n = 6) |
| Control + aristolochic acid | 4.5 ± 0.1 (n = 6) |
| Elicitor + aristolochic acid | 5.5 ± 0.2 (n = 4) |
Fluorescence developed within 2 min of incubation with the fluorogenic substrate BPC in independent experiments with 3-day-old cell suspensions. Inhibitor concentrations were 10 μM ETYA and 20 μM aristolochic acid.