Figure 3.

Kinetics of the Formation of Nuclear Speckles in Transgenic Arabidopsis Seedlings Expressing phyA to phyE:GFP Fusion Proteins.

Seven-day-old dark-adapted seedlings were irradiated for 8 h with FR (B) or WL ([A] and [C] to [F]), and nuclei of hypocotyl cells were analyzed by epifluorescence microscopy at the times shown. The values reflect the relative numbers of nuclei containing phyA:GFP ([A] and [B]), phyB:GFP (C), phyC:GFP (D), phyD:GFP (E), and phyE:GFP (F) intranuclear speckles. For each time point, at least three independent experiments were performed. In a single experiment, ∼80 nuclei were analyzed in five different seedlings.