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Fusion Protein Constructs for the Split-Ubiquitin System.
Interactions of LeSUT2-CubPLV were determined with all of the other proteins shown, and interactions of LeSUT1-CubPLV were determined with the other Suc transporter fusion constructs. For the constructs, open reading frames were amplified by PCR and cloned into the expression vectors in frame with the C-terminal (Cub) or the N-terminal (Nub) half of ubiquitin. Restriction sites important for cloning are indicated.
